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Quantitation: Enhancement Assays

Enhancement assays require the use of two binding partners. Capture receptors (RC) are fixed onto the unclad optical fiber while detection receptors (RD) are bound to the AuNPs. During a sensing event, RD-functionalized AuNPs are introduced to the RC-functionalized sensing surface with the target analytes (A). AuNPs are brought to the sensing surface through formation of sandwich-like RD-A-RC complexes. Presence of AuNPs at the sensing surface results in a dramatic optical response.

Target: procalcitonin (PCT) in serum

Septicemia is a life-threatening condition caused by bacterial infection of the blood. This may lead to sepsis, a serious complication that causes inflammation throughout the body, resulting in blood clots to cause organ failure. Procalcitonin (PCT) is a biomarker that can specifically diagnose septicemia and measure its severity with high accuracy.​​​​​​​

Anti-PCT capture antibodies are immobilized onto the sensing surface while anti-PCT detection antibodies are functionalized onto the AuNPs. During detection, presence of PCT results in formation of sandwich-like antibody-antigen-antibody-AuNP conjugates at the sensing surface.