Quantitation: Enhancement Assays
Enhancement assays require the use of two binding partners. Capture receptors (RC) are fixed onto the unclad optical fiber while detection receptors (RD) are bound to the AuNPs. During a sensing event, RD-functionalized AuNPs are introduced to the RC-functionalized sensing surface with the target analytes (A). AuNPs are brought to the sensing surface through the formation of sandwich-like RD-A-RC complexes. The presence of AuNPs at the sensing surface results in a dramatic optical response.

定量：增強分析
增強分析法需要使用兩種結合對象，捕獲受體(RC)被固定在未包裹的光纖上，而檢測受體(RD)則被綁定到金奈米粒子(AuNPs)上。在檢測時，RD功能化的AuNPs與目標分析物(A)一同引入到功能化RC的感測表面上，形成類似三明治狀的RD-A-RC複合物。於感測表面上，AuNPs的存在會產生劇烈的光學反應，進而執行偵測。

Target: procalcitonin (PCT) in serum
Septicemia is a blood poisoning condition caused by bacterial infection of the blood. This may lead to sepsis, a serious complication that causes inflammation throughout the body, which may result in blood clot formation and subsequent organ failure. Procalcitonin (PCT) is a biomarker that can specifically diagnose septicemia and measure its severity with high accuracy.​​​​​​​

目標：血清中的降鈣素(PCT)
敗血症是由細菌感染血液引起的血液汙染狀態，這可能會導致敗毒症(sepsis)，一種引起全身發炎的嚴重併發症，可能導致血栓形成，進而引起器官功能衰竭。降鈣素原（PCT）是一種生物標誌物，可以特異性地診斷敗血症，並高度準確地測量其嚴重程度。

Anti-PCT capture antibodies are immobilized onto the sensing surface while anti-PCT detection antibodies are functionalized onto the AuNPs. During detection, the presence of PCT results in the formation of sandwich-like antibody-antigen-antibody-AuNP conjugates at the sensing surface.

抗降鈣素原捕獲抗體被固定在感測表面，而抗降鈣素原檢測抗體則被功能化到金奈米粒子上。在檢測過程中，抗降鈣素原的存在會導致三明治狀的抗體-抗原-抗體-金奈米粒子共軛結構在感測表面形成。